細胞培養支原體污染：發(fā)生率，來(lái)源，效果，檢測，消除，預防

細胞培養物由支原體污染保持在細胞培養物中的一個(gè)主要問(wèn)題。支原體能產(chǎn)生無(wú)限種在他們感染的培養影響。本文締一生物/締一生物為您分析細胞培養支原體污染：發(fā)生率，來(lái)源，效果，檢測，消除，預防。

　　這些微生物是在細胞培養中常用的多數抗生素抗性。在這里，我們提供關(guān)于當前知識的一簡(jiǎn)要的概述：

（1）發(fā)生率和支原體污染的在細胞培養物來(lái)源，支原體物種在細胞培養物zui常檢測到的，和支原體的上的功能和被感染細胞培養物的影響的活動(dòng);

（2）可用于檢測特別強調zui可靠的檢測方法支原體的各種技術(shù);

（3）可用于消除支原體突出抗生素治療的各種方法;

（4）推薦用于支原體污染的檢測，消除和預防程序和工作協(xié)議。準確，靈敏和可靠的檢測方法的可用性和健壯和成功的消除方法的應用提供用于克服在細胞培養物的支原體污染問(wèn)題的有力手段。

英文原文：

The contamination of cell cultures by mycoplasmas remains a major problem in cell culture.Mycoplasmas can produce a virtually unlimited variety of effects in the cultures they infect. These organisms are resistant to most antibiotics commonly employed in cell cultures.

Here we provide a concise overview of the current knowledge on:

(1) the incidence and sources of mycoplasma contamination in cell cultures, the mycoplasma species most commonly detected in cell cultures, and the effects of mycoplasmas on the function and activities of infected cell cultures;

(2) the various techniques available for the detection of mycoplasmas with particular emphasis on the most reliable detection methods;

(3) the various methods available for the elimination of mycoplasmas highlighting antibiotic treatment;

(4) the recommended procedures and working protocols for the detection, elimination and prevention of mycoplasma contamination. The availability of accurate, sensitive and reliable detection methods and the application of robust and successful elimination methods provide powerful means for overcoming the problem of mycoplasma contamination in cell cultures.

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